Nanchangia Anserum Gen. Nov., Sp. Nov., Isolated from Feces of Greater White-Fronted Geese (anser Albifrons).

Two rod-shaped and Gram-stain-positive bacteria (strains C64(T) and C62) were isolated in 2020 from faeces of greater white-fronted geese (Anser albifrons) from Poyang Lake, PR China. Their optimal growth conditions were at 37 degrees C, pH 7.0 and with 0.5% (w/v) NaCl. The two isolates showed a highest 16S rRNA gene sequence similarity to Bowdeniella nasicola DSM 19116(T) (92.1%). Phylogenetic/phylogenomic analyses indicated that strains C64(T) and C62 clustered independently in the vicinity of the genera Varibaculum, Winkia and Mobiluncus within the family Actinomycetaceae, but could not be classified clearly as members of any of these known genera. The average amino acid identity values between our isolates and available genomes of members of the family Actinomycetaceae were around the genus threshold value (45-65%). The major cellular fatty acids of the strains were C-18:1 omega 9c and C-16:0. The predominant polar lipids were phosphatidylinositol, phosphatidylglycerol, phosphatidylcholine, diacylglycerol, triacylglycerol and cardiolipin. The amino acid composition of peptidoglycan contained alanine, glutamic acid and glycine. The major respiratory menaquinones were MK-8(H-4) and MK-9(H-4). The whole cell sugars included galactose, arabinose and glucose. On the basis of the results of the 165 rRNA gene sequences comparison. whole-genome phylogenomic analysis. phenotypic and chemotaxonomic characteristics, we propose that strains C64(T) and C62 represent a novel species belonging to a novel genus within the family Actinomycetaceae, for which the name Nanchangia anserum gen. nov., sp. nov. is proposed. The type strain is Nanchangia anserum C64(T) (=CGMCC 1.18410(T)=GDMCC 1.1969(T)=KCTC 49511(T)=KACC 22143(T)).