Author Response for "A Chitinase Involved in the Mycoparasitism of Trichoderma Atroviride on Aeciospores of Cronartium Ribicola"

Trichoderma atroviride SS003 is a mycoparasite of Cronartium ribicola, and it has a significant destructive effect on rust aeciospores. Based on transcriptome sequencing results and qPCR validation, the expression of a chitinase gene named CHI10 was upregulated after induction for 24, 72 and 120 h. A 1.3-kb cDNA of CHI10 was obtained by RT-PCR from SS003 mycelium co-cultured with the cell walls of the aeciospores for 24 h. After the prokaryotic expression of CHI10 gene in Escherichia coli, a recombinant 46.0 kD protein was obtained, and the target fusion protein was partially purified, which was shown to have destructive effects on the cell wall of aeciospores at 40-55 degrees C. This chitinase may be associated with the mycoparasitism of T. atroviride on aeciospores of C. ribicola.