High Spatial Resolution Correlative Imaging of Cryo-SXT and GSDIM for Identification of Three-Dimensional Subcellular Structures

Correlative imaging of soft X-ray tomography (SXT) and fluorescence microscopy (FM) has emerged as a promising strategy to provide complementary morphological and functional information. Despite much progress achieved in correlative imaging, precise identification of three-dimensional subcellular structures inside cells needs to be improved. Here, we present a high-resolution correlative imaging method by coupling ground state depletion microscopy followed by individual molecule return (GSDIM) and Cryo-soft X-ray tomography (Cryo-SXT). The custom-designed correlative imaging enables to provide high spatial resolution fusion image of three-dimensional subcellular structure inside cell with depth of several micrometers. Furthermore, the GSDIM is facile, cost-effective and maneuverable. We believe this advanced technique would be a powerful imaging toolkit to provide useful and comprehensive information in bioscience.