Description of Corynebacterium Poyangense Sp. Nov., Isolated from the Feces of the Greater White-Fronted Geese ( Anser Albifrons )

Two novel Gram-positive, non-spore-forming, facultatively anaerobic, non-motile, and short rods to coccoid strains were isolated from the feces of the greater white-fronted geese ( Anser albifrons ) at Poyang Lake. The 16S rRNA gene sequences of strains 4H37-19 T and 3HC-13 shared highest identity to that of Corynebacterium uropygiale Iso10 T (97.8%). Phylogenetic and phylogenomic analyses indicated that strains 4H37-19 T and 3HC-13 formed an independent clade within genus Corynebacterium and clustered with Corynebacterium uropygiale Iso10 T . The average nucleotide identity and digital DNA-DNA hybridization value between strains 4H37-19 T and 3HC-13 and members within genus Corynebacterium were all below 95% and 70%, respectively. The genomic G + C content of strains 4H37-19 T and 3HC-13 was 52.5%. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylcholine, and phosphatidyl inositol mannosides (PIM) were the major polar lipids, with C 18:1 ω 9 c , C 16:0 , and C 18:0 as the major fatty acids, and MK-8 (H4), MK-8(H 2 ), and MK-9(H 2 ) as the predominant respiratory quinones. The major whole cell sugar was arabinose, and the cell wall included mycolic acids. The cell wall peptidoglycan contained meso -diaminopimelic acid ( meso -DAP). The polyphasic taxonomic data shows that these two strains represent a novel species of the genus Corynebacterium , for which the name Corynebacterium poyangense sp. nov. is proposed. The type strain of Corynebacterium poyangense is 4H37-19 T (=GDMCC 1.1738 T = KACC 21671 T ).