Supplementary Materials and Methods, Figures 1 - 14, Table 1 from <i>PCAT-1</i>, a Long Noncoding RNA, Regulates BRCA2 and Controls Homologous Recombination in Cancer

PDF file - 2687KB, Supplementary Figure S1: Effect of PCAT-1 on RAD51 foci formation. Supplementary Figure S2: PCAT-1 impairs homologous recombination. Supplementary Figure S3: PCAT-1 expression alters gamma-H2AX formation. Supplementary Figure S4: PCAT-1 expression effects clonogenic survival when treated with PARP inhibitors Olaparib or ABT-888. Supplementary Figure S5: PCAT-1 expression effects clonogenic survival when treated with ionizing radiation. Supplementary Figure S6: Treatment of PCAT-1 isogenic cells with ABT-888. Supplementary Figure S7: PCAT-1 overexpression in RWPE engenders sensitivity to genotoxic stress. Supplementary Figure 8: BRCA2 knockdown rescues RAD51 foci formation in LNCAP shPCAT1 cells after PARP inhibition. Supplementary Figure 9: PCAT-1 expression does not impact cell cycle distribution. Supplementary Figure S10: Effect of PCAT-1 in vivo on Olaparib treatment. Supplementary Figure S11: PCAT-1 does not operate via an epigenetic or STAU1-based mechanism. Supplementary Figure 12: PCAT-1 is cytoplasmic and effects target mRNA stability. Supplementary Figure 13: PCAT-1 overexpression in RWPE cells. Supplementary Figure 14: A model of PCAT-1 function in homologous recombination in prostate cancer. Supplementary Table 1: Primer Pairs used in this study.