The Impact of Early Life Exposure to Individual and Combined Pfas on Learning, Memory, and Bioaccumulation in C. Elegans

Per- and Polyfluoroalkyl Substances (PFAS) are a group of water-soluble chemicals used for decades with important industrial and commercial applications. Due to their chemical and thermal stability, persistence in the environment, and widespread human exposure, PFAS become an important concern for public health. In this study, eleven highly prevalent PFAS and a reference mixture were selected according to various drinking water sources. The nematode, Caenorhabditis elegans, were exposed to PFAS at 0.1, 1, 10, 100, and 200 mu M, and the toxic effects on learning & memory along with the bioaccumulation were investigated using a high-throughput screening (HTS) platform. Our results showed that perfluorooctanesulfonic acid (PFOS) and perfluorobutanesulfonic acid (PFBS) exhibited significant inhibitory effects (p < 0.05) on learning and memory in both time points at concentrations between 100 and 200 mu mol/L. After 48 h of exposure, every PFAS resulted in an inhibition of learning and memory with a concentration of 200 mu mol/L. Furthermore, the PFOS and PFBS had the highest bioaccumulation levels after 48 h of exposure. These findings provide valuable insight into the developmental adverse effects associated with exposure and the bioaccumulation of both individual and mixtures of PFAS.