Superenhancer-driven Circrna Myst4 Involves in Pulmonary Artery Smooth Muscle Cell Ferroptosis in Pulmonary Hypertension

The abnormal expression of circular RNAs (circRNAs) is emerging as a critical cause in regulation of pathological changes of hypoxic pulmonary hypertension (PH), in which ferroptosis is a new pathological a significant decrease in circMyst4 expression in hypoxia. In vitro assays revealed that circMyst4 alleviated the nucleus to promote GPX4 mRNA processing and inhibiting the formation of the Eef1a1/ACSL4 complex in the cytoplasm. Additionally, superenhancer (SE) was verified to drive the generation of circMyst4. In vivo assays revealed that circMyst4 inhibited the progression of hypoxic PH. Overall, SE-driven circDDX5-regulated GPX4 mRNA processing and inhibiting the binding between Eef1a1 and ACSL4.